What Do Pets Need

How to cycle a new fish tank

Cycling grows the bacteria in a new filter that turn fish waste into nitrate, which is safer. Run the tank without fish, test the water, and add fish only once ammonia and nitrite stay at zero.

How long it takes

The sources don’t agree, and RSPCA says there’s no exact rule. Plan for the longest and let the tests decide.

How long cycling takes, by source
SourceHow longIn its words
RSPCAAt least two weeks“There is no exact rule for how long cycling will take but it will be at least two weeks.”
RSPCA AustraliaUsually 4 to 6 weeksFully cycled once nitrates are being produced and ammonia and nitrite are zero, which “usually takes 4 to 6 weeks”.
UF/IFAS ExtensionSix to eight weeks“A new biofilter requires six to eight weeks to build up sufficient bacteria to effectively reduce ammonia and nitrite levels.”
OATASeveral weeksThe fishless method adds “a calculated dose of ammonia to your aquarium over several weeks”.

Steps

  1. Set up and wait. Fill the tank and start the filter. OATA advises leaving a new aquarium 24 to 48 hours to settle.
  2. Cycle without fish. RSPCA strongly advises new owners to use fishless cycling, running the filter for a while before any fish go in. OATA’s fishless method adds a calculated dose of ammonia over several weeks. It says cycling with a few hardy fish should only be done with great care and a shop’s guidance.
  3. Test the water. Test for ammonia and nitrite every other day (RSPCA), or every two to three days for the first six weeks (RSPCA Australia).
  4. Watch the numbers. Ammonia and nitrite rise and then fall, while nitrate usually keeps rising (OATA).
  5. Know when it’s done. The filter is mature once ammonia and nitrite have dropped to zero and stay at zero (OATA). RSPCA adds fish only after a spike in both, followed by a return to zero.
  6. Add fish slowly. Don’t add a lot of fish at once: RSPCA Australia says gradual introduction lets the filter bacteria adapt.

Why it matters

RSPCA Australia says the number one cause of fish deaths in new aquariums is a filter without enough bacteria, letting ammonia build up. It says ammonia is toxic to fish at 0.1 ppm, and UF/IFAS Extension says nitrite is toxic at levels as low as 0.10 mg per liter.

UF/IFAS adds that the un-ionized form of ammonia is about 100 times more toxic than the ionized form, and that its share rises as pH or temperature rises.

Once it’s cycled

Keep testing and change some water every week: RSPCA says 30% weekly, and OATA up to 25% every week. When you clean the filter, never rinse its media under a tap, which washes away and kills the bacteria (OATA).

Common questions

Can I add fish on the first day?

No. OATA advises letting a new aquarium settle for 24 to 48 hours, and RSPCA says to add fish only once ammonia and nitrite have spiked and come back down to zero.

What is new tank syndrome?

OATA says overstocking a tank, or stocking it too quickly, can result in new tank syndrome.

What should ammonia and nitrite read?

Zero. RSPCA gives 0 mg per liter for both ammonia and nitrite in a fish tank.

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Where we get our information from

The steps and times come from OATA, RSPCA, RSPCA Australia and the University of Florida’s IFAS Extension. Checked against published data from RSPCA, OATA, RSPCA Australia and UF/IFAS Extension.

See all 4 sources
  • RSPCA, Ornamental fish: Environment (“Choosing an aquarium”)
  • OATA, How to set up and maintain a freshwater aquarium, September 2022 (PDF)
  • RSPCA Australia knowledgebase, Why is water quality important when setting up a fish aquarium?
  • University of Florida IFAS Extension, FA16 “Ammonia in Aquatic Systems” (2022 revision, PDF)

General care guidance from animal welfare charities, vets and makers' published sizes. It isn't veterinary advice: if your pet seems unwell or has eaten something it shouldn't, call your vet.